POLIB Antibody

Code CSB-PA775623XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) POLIB Polyclonal antibody
Uniprot No.
Target Names
POLIB
Alternative Names
POLIB antibody; At3g20540 antibody; K10D20.8 antibody; DNA polymerase I B antibody; chloroplastic/mitochondrial antibody; EC 2.7.7.7 antibody; DNA polymerase PolI-like B antibody; AtPolI-like B antibody; Polymerase gamma 1 antibody; POLGAMMA1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana POLIB protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity. Required for DNA replication and accumulation in plastids and mitochondria.
Gene References into Functions
  1. PolIA & PolIB are functionally equipped to play a role in short-patch BER and suggest a major role of PolIA & PolIB in a predicted long-patch base excision repair (BER) sub-pathway. We propose that the acquisition of insertion 1 in the polymerization domain of PolIA & PolIB was a key component in their evolution as BER associated and replicative DNA polymerases. PMID: 29522990
  2. both AtPolIA and AtPolIB are efficient translesion DNA synthesis polymerases, providing the first example of a family-A DNAP with an active exonuclease domain that efficiently bypasses an AP site. PMID: 28977655
  3. results suggest that polIB (At3G29540) mutants undergo an adjustment in cell homeostasis, enabling them to maintain functional mitochondria at the cost of normal cell expansion and plant growth PMID: 23167278
  4. UV-B irradiation enhances expression of AtPollambda in wild-type Arabidopsis. Overexpression partially complements DNA damage repair efficiency of atpollambda mutants. PMID: 21227935
Subcellular Location
Mitochondrion. Plastid, chloroplast.
Protein Families
DNA polymerase type-A family
Tissue Specificity
Expressed in shoot apical meristem.
Database Links

KEGG: ath:AT3G20540

STRING: 3702.AT3G20540.2

UniGene: At.38221

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