PORB Antibody

Code CSB-PA324147XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PORB Polyclonal antibody
Uniprot No.
Target Names
PORB
Alternative Names
PORB antibody; At4g27440 antibody; F27G19.40 antibody; Protochlorophyllide reductase B antibody; chloroplastic antibody; PCR B antibody; EC 1.3.1.33 antibody; NADPH-protochlorophyllide oxidoreductase B antibody; POR B antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PORB protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Gene References into Functions
  1. reconstituted Pchlide-POR-NADPH complexes showed fluorescence maxima at 77 K PMID: 25659137
  2. In response to illumination, light-dependent chlorophyll production, thylakoid stacking and photomorphogenesis are also restored in PORA-overexpressing porB-1 porC-1 seedlings and adult plants. PMID: 20012672
  3. The analysis of PORA and PORB import into plastids of intact seedlings revealed an unexpected multiplicity of import routes that differed by their substrate, cell, tissue and organ specificities. PMID: 15842619
Subcellular Location
Plastid, chloroplast outer membrane. Plastid, chloroplast thylakoid membrane.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family, POR subfamily
Tissue Specificity
Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Expressed in seedlings and adult plant.
Database Links

KEGG: ath:AT4G27440

STRING: 3702.AT4G27440.1

UniGene: At.23416

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