POT5 Antibody

Code CSB-PA862994XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) POT5 Polyclonal antibody
Uniprot No.
Target Names
POT5
Alternative Names
POT5 antibody; HAK5 antibody; At4g13420 antibody; T9E8.160 antibody; Potassium transporter 5 antibody; AtHAK1 antibody; AtHAK5 antibody; AtPOT5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana POT5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
High-affinity potassium transporter. Can also transport rubidium and cesium (Ref.1). Is essential with AKT1 for high-affinity potassium uptake in roots during seedling establishment and postgermination growth under low potassium conditions. Mediates potassium uptake by plant roots in response to low potassium conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by the calcium sensors calcineurin B-like genes CBL1, CBL8, CBL9 and CBL10, and by phosphorylation by CIPK23.
Gene References into Functions
  1. results demonstrate that ARF2 plays important roles in the response to external K(+) supply in Arabidopsis and regulates HAK5 transcription accordingly. PMID: 27895227
  2. ILK1 interacted with and promoted the accumulation of HAK5, a putative (H(+))/K(+) symporter that mediates a high-affinity uptake during K(+) deficiency.[ILK1] PMID: 27208244
  3. Data show that Arabidopsis Mutants lacking CBL-interacting protein kinase23 (CIPK23) are deficient in high-affinity K(+) transporter5 (HAK5)-mediated high-affinity potassium (K(+)) uptake. PMID: 26474642
  4. On the one hand the existence of a posttranscriptional regulation of HAK5 elicited by the low K(+) signal and on the other that HAK5 may be involved in yet-unknown functions related to NO(3)(-) and P deficiencies. PMID: 24716623
  5. Lack of ETO1 function leads to significant enhancement of tissue K status by an RBOHF-independent mechanism associated with elevated high-affinity K(+) TRANSPORTER5 transcript levels. PMID: 24064768
  6. HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress. PMID: 23825216
  7. Although having a limited capacity, AtHAK5 plays a major role for K(+) acquisition from low K(+) concentrations in the presence of salinity. PMID: 20028724
  8. Results suggested that RCI3 is involved in the production of ROS under potassium deprivation and that RCI3-mediated ROS production affects the regulation of AtHAK5 expression. PMID: 20139158
  9. AtHAK5 is the only system mediating K+ uptake at concentrations below 0.01 mM. In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. PMID: 20088908
  10. Upregulation of the HAK5 protein in response to potassium deprivation in A. thaliana. PMID: 15734909
  11. AtHAK5 is a root plasma membrane uptake mechanism for K(+) and Cs(+) under conditions of low K(+) availability. PMID: 18281719
  12. AtHAK5 mediates K+ absorption at low concentration better than AtAKT1. AtHAK5 induction is repressed by NH4+. PMID: 19000196

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
HAK/KUP transporter (TC 2.A.72.3) family
Tissue Specificity
Predominantly expressed in the roots.
Database Links

KEGG: ath:AT4G13420

STRING: 3702.AT4G13420.1

UniGene: At.3067

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