PCMP-A2 Antibody

Code CSB-PA604495XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PCMP-A2 Polyclonal antibody
Uniprot No.
Target Names
PCMP-A2
Alternative Names
PCMP-A2 antibody; PDM1 antibody; SEL1 antibody; At4g18520 antibody; F28J12.180 antibody; Pentatricopeptide repeat-containing protein At4g18520 antibody; chloroplastic antibody; Protein PIGMENT-DEFICIENT MUTANT 1 antibody; Protein SEEDLING LETHAL 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PCMP-A2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for proper chloroplast development (Ref.7, PubMed:24144791). Involved in the regulation of plastid gene expression probably through regulation of plastid-encoded polymerase (PEP) dependent chloroplast transcription. Required for RNA editing of several chloroplastic transcripts, especially accD transcripts. Required for processing of the chloroplastic rpoA pre-mRNA (Ref.6, Ref.7). Required for the monocistronic rpoA transcript processing from the rpl23-rpl2-rps19-rpl22-rps3-rpl16-rpl14-rps8-rpl36-rps11-rpoA polycistron. Binds the intergenic sequence of rps11-rpoA for rpoA monocistronic RNA cleavage (Ref.7).
Gene References into Functions
  1. PDM1 is involved in RNA editing and splicing. Therefore, PDM1 is an important protein for post-transcriptional regulation in chloroplast. PMID: 26123918
  2. SEL1 is involved in the regulation of plastid gene expression required for normal chloroplast development.[SEL1] PMID: 24144791
Subcellular Location
Plastid, chloroplast.
Protein Families
PPR family, PCMP-A subfamily
Tissue Specificity
Expressed specifically in aerial greening tissues, such as cotyledons, rosette leaves, cauline leaves, stems, sepals, stamens, carpels and siliques.
Database Links

KEGG: ath:AT4G18520

STRING: 3702.AT4G18520.1

UniGene: At.32894

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