pph-4.1 Antibody

Code CSB-PA893624XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans pph-4.1 Polyclonal antibody
Uniprot No.
Target Names
pph-4.1
Alternative Names
pph-4.1 antibody; Y75B8A.30 antibody; Serine/threonine-protein phosphatase 4 catalytic subunit 1 antibody; PP4C-1 antibody; EC 3.1.3.16 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans pph-4.1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein phosphatase which plays an essential role in meiosis and in early embryonic mitosis. During spermatocyte meiosis and the first embryonic mitosis, regulates centrosome maturation, and thus spindle formation, by recruiting some of the components of the pericentriolar material (PCM). During oocyte meiosis I, regulates meiotic chromosome dynamics including synapsis-independent chromosome pairing, restriction of synapsis to homologous chromosomes, programmed DNA double-strand break initiation and crossover formation resulting in chiasma formation. During oocyte meiosis II and probably together with regulatory subunit ppfr-1, may regulate microtubule severing by dephosphorylating and activating mei-1, a component of the katanin microtubule severing complex.
Gene References into Functions
  1. We show that in Caenorhabditis elegans, PPH-4.1, is required independently to carry out four separate functions involving meiotic chromosome dynamics PMID: 25340746
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
PPP phosphatase family, PP-4 (PP-X) subfamily
Database Links
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