flfl Antibody

Code CSB-PA893273XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) flfl Polyclonal antibody
Uniprot No.
Target Names
flfl
Alternative Names
flfl antibody; CG9351 antibody; Serine/threonine-protein phosphatase 4 regulatory subunit 3 antibody; PP4R3 antibody; Protein falafel antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) flfl protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulatory subunit of serine/threonine-protein phosphatase 4. The probable PP4 complex Pp4-19C-PPP4R2r-flfl (PPP4C-PPP4R2-PPP4R3) is required to prevent caspase induced cell death (in vitro). May be involved in DNA damage repair. Key mediator specific for the localization of mira and associated cell fate determinants during both interphase and mitosis. Nuclear Flfl is required to exclude mira/pros from the nucleus when inefficiently bound to the cytoskeleton/cortex, whereas cytosolic or membrane-associated flfl is required for the cortical association and asymmetric localization of mira/pros/brat/stau at metaphase and anaphase.
Gene References into Functions
  1. These data report for the first time an essential physiological function of flfl in maintaining tissue homeostasis and organ development PMID: 26583122
  2. identified the evolutionarily conserved Protein Phosphatase 4 (PP4) regulatory subunit PP4R3/Falafel (Flfl) as a key mediator specific for the localization of Miranda (Mira) and associated cell fate determinants during both interphase and mitosis PMID: 19204120
Subcellular Location
Nucleus. Membrane. Cytoplasm. Note=Predominantly nuclear during interphase/prophase and cytoplasmic after nuclear envelope breakdown.
Protein Families
SMEK family
Tissue Specificity
Expressed in neuroblasts.
Database Links

KEGG: dme:Dmel_CG9351

STRING: 7227.FBpp0082285

UniGene: Dm.14510

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