Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PHO5 Polyclonal antibody

Uniprot No.

P00635

Target Names

PHO5

Alternative Names

PHO5 antibody; YBR093C antibody; YBR0814 antibody; Repressible acid phosphatase antibody; EC 3.1.3.2 antibody; P60 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PHO5 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Partially mediates extracellular nucleotide derived phosphate hydrolysis along with NPP1 and NPP2.

Gene References into Functions

A review of the PHO5 promoter as a paradigm for gene regulation through chromatin remodeling. PMID: 25190457
The results show that remodels the structure of chromatin (RSC) is crucially involved in PHO5 promoter opening. PMID: 24465003
we provide evidence that topoisomerases are required to maintain the PHO5 promoter in a superhelical state, which is competent for proper activation. PMID: 23284296
data suggest that Set1-mediated H3K4me suppresses nucleosome remodeling at the PHO5 promoter so as to reduce basal transcription of PHO5 under repressive conditions PMID: 21646424
auxiliary role for DNA binding competition between Pho4 and histones in PHO5 promoter chromatin remodeling PMID: 20566699
YAF9 poises the PHO5 promoter for chromatin remodeling and activation. PMID: 15175650
These results support a probabilistic model in which chromatin remodeling at PHO5 spreads from sites of transactivator association with DNA and attenuates with distance. PMID: 16491089
Chromatin diassembly from the PHO5 promoter is essential for the recruitment of the general transcription machinery and coactivators. PMID: 17620413
analysis of complete independence of promoter chromatin opening from downstream PHO5 transcription processes PMID: 17631505
Nap1 regulates transcription of PHO5. PMID: 18227150
The Mcm1-Fkh2 complex, first shown to transactivate genes within the CLB2 cluster that drive G(2)/M progression, also associated directly at the PHO5 promoter in a cell cycle-dependent manner. PMID: 19596791

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Subcellular Location

Secreted.

Protein Families

Histidine acid phosphatase family

Database Links

KEGG: sce:YBR093C

STRING: 4932.YBR093C

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PHO5 Antibody

Product Details

Customer Reviews and Q&A

Target Background