PPDK Antibody

Datasheet

Code	CSB-PA515808XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PPDK Polyclonal antibody

Uniprot No.

O23404

Target Names

PPDK

Alternative Names

PPDK antibody; At4g15530 antibody; dl3805c antibody; FCAALL.325 antibody; Pyruvate antibody; phosphate dikinase 1 antibody; chloroplastic antibody; EC 2.7.9.1 antibody; Pyruvate antibody; orthophosphate dikinase 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PPDK protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Formation of phosphoenolpyruvate. May be involved in regulating the flux of carbon into starch and fatty acids of seeds and in the remobilization of nitrogen reserves in senescing leaves.

Gene References into Functions

Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment, one is via PCK1, and a second via PPDK. PMID: 25858700
Over-expression of PPDK during senescence increases nitrogen remobilization from senescing leaves and increases seed size and seed nitrogen content. PMID: 20202167
In cotyledons pyruvate orthophosphate dikinase may be important in supplying phosphoenol pyruvate to gluconeogenesis, and in ageing leaves it allows remobilisation of nitrogen to supply reproductive tissue [pyruvate orthophosphate dikinase] [PPDK] PMID: 16915520

Subcellular Location

Plastid, chloroplast. Cytoplasm. Note=Isoform 1 is targeted to the chloroplast while isoform 2 is found in the cytoplasm.

Protein Families

PEP-utilizing enzyme family

Tissue Specificity

Isoform 1 is expressed in leaves, flowers and siliques. Isoform 2 is found in cotyledons, rosette and cauline leaves, petioles, flowers and siliques.

Database Links

KEGG: ath:AT4G15530

STRING: 3702.AT4G15530.5

UniGene: At.23286

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PPDK Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background