PPI1 Antibody

Datasheet

Code	CSB-PA828999XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PPI1 Polyclonal antibody

Uniprot No.

O23144

Target Names

PPI1

Alternative Names

PPI1 antibody; At4g27500 antibody; F27G19.100 antibody; Proton pump-interactor 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PPI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Promotes AHA1 plasma membrane ATPase activity by binding to a site different from the 14-3-3 binding site.

Gene References into Functions

PPI1 can bind the H(+)-ATPase independently of the C-terminus conformation, but is not able to suppress the C-terminus auto-inhibitory action. PMID: 16279950
Data show that Ppi1 expression is strong in root and shoot vascular systems, particularly in meristematic and sink tissues, as well as in pollen, stigmas and siliques, but not in developing embryos. PMID: 18304198
PPI1 is localised at the endoplasmic reticulum, from which it is recruited to the plasma membrane for interaction with H(+)-ATPase. PMID: 19796364

Subcellular Location

Cell membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

Plant Proton pump-interactor protein family

Tissue Specificity

Strongly expressed in root and shoot vascular systems, particularly in meristematic and sink tissues. Also present in pollen, stigmas and siliques, but not in developing embryos.

Database Links

KEGG: ath:AT4G27500

STRING: 3702.AT4G27500.1

UniGene: At.3381

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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