PPO2 Antibody

Code CSB-PA894645XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) PPO2 Polyclonal antibody
Uniprot No.
Target Names
PPO2
Alternative Names
PPO2 antibody; proPo-A3 antibody; proPO45 antibody; CG8193 antibody; Phenoloxidase 2 antibody; PO A3 antibody; EC 1.14.18.1 antibody; Phenoloxidase subunit A3 antibody; Processed phenoloxidase A3 antibody; Pro-phenoloxidase A3 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) PPO2 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6 dihydroxyindole to indole-5'6 quinonee.
Gene References into Functions
  1. PPO1 and PPO2 contribute to the bulk of melanization in the hemolymph, upon septic or clean injury. In contrast, PPO3, is expressed in lamellocytes and contributes to melanization during the encapsulation process. PMID: 26437768
  2. An important role for PPO1 and PPO2 in the survival to infection with Gram-positive bacteria and fungi, underlining the importance of melanization in insect host defense. PMID: 24788090
  3. rPPO1 and rPPO2 were expressed as apo-rPPO and became holo-PPO upon Cu(2+) binding; rPPO3 was holo-PPO immediately after expression. PMID: 22178920
  4. biochemical properties, activation mode and in vivo role of Pro-PO3 (PPO3) are likely distinct from those of the other two Pro-PO enzymes involved in Drosophila physiology. PMID: 18852525
Subcellular Location
Secreted. Note=Expressed in larval hemolymph.
Protein Families
Tyrosinase family
Database Links

KEGG: dme:Dmel_CG8193

STRING: 7227.FBpp0087744

UniGene: Dm.538

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