PREP1 Antibody

Datasheet

Code	CSB-PA864777XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PREP1 Polyclonal antibody

Uniprot No.

Q9LJL3

Target Names

PREP1

Alternative Names

PREP1 antibody; ZNMP1 antibody; At3g19170 antibody; MVI11.6 antibody; MVI11.7 antibody; MVI11_8Presequence protease 1 antibody; chloroplastic/mitochondrial antibody; AtPreP1 antibody; PreP 1 antibody; EC 3.4.24.- antibody; Zinc metalloprotease 1 antibody; AtZnMP1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PREP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, with a bias for positively charged amino acid residues.

Gene References into Functions

Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species. PMID: 21621546
AtPreP functions as a peptidasome that degrades targeting peptides and other small unstructured peptides. In addition to Zn located in the catalytic site, AtPreP also contains two Mg-binding sites. [PreP1] PMID: 19646442
AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis PMID: 17081117
The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development. PMID: 19701724
AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. AtPrePI showed a preference for the N-terminal amphiphilic α-helix and positively charged amino acid residues. PMID: 15827031

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Subcellular Location

Plastid, chloroplast stroma. Mitochondrion matrix.

Protein Families

Peptidase M16 family, PreP subfamily

Tissue Specificity

Expressed only in siliques and flowers.

Database Links

KEGG: ath:AT3G19170

STRING: 3702.AT3G19170.1

UniGene: At.27915

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PREP1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background