PRK1 Antibody

Datasheet

Code	CSB-PA340260XA01SVG
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Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PRK1 Polyclonal antibody

Uniprot No.

P40494

Target Names

PRK1

Alternative Names

PRK1 antibody; PAK1 antibody; YIL095W antibody; Actin-regulating kinase PRK1 antibody; EC 2.7.11.1 antibody; p53-regulating kinase 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PRK1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Protein kinase involved in the regulation of actin cytoskeleton organization and endocytosis. Phosphorylates PAN1 which disrupts the interaction between PAN1 and END3, and between PAN1 and SLA1. Phosphorylates SCD5. Preferentially, phosphorylates substrates on threonine residues in a [L/I/V/M]-x-x-[Q/N/T/S]-x-T-G motif.

Gene References into Functions

Data show that Sjl2p is specifically associated with cortical actin patches which aggregate upon loss of the actin-regulating kinases Ark1p and Prk1p. PMID: 16406366
Overexpressed Prk1 conferred Adriamycin resistance in yeast cells. PMID: 17178891
autophosphorylation of Prk1p may represent a feedback mechanism possibly involved in fine-tuning the pace of progression during actin-coupled endocytosis PMID: 18939955

Subcellular Location

Cytoplasm, cytoskeleton, actin patch. Note=Cortical actin patches.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: sce:YIL095W

STRING: 4932.YIL095W

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PRK1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background