prmC Antibody

Datasheet

Code	CSB-PA364776XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) prmC Polyclonal antibody

Uniprot No.

P0ACC1

Target Names

prmC

Alternative Names

prmC antibody; hemK antibody; b1212 antibody; JW1203 antibody; Release factor glutamine methyltransferase antibody; RF MTase antibody; EC 2.1.1.297 antibody; M.EcoKHemKP antibody; N5-glutamine methyltransferase PrmC antibody; Protein release factor methylation C antibody; Protein-(glutamine-N5) MTase PrmC antibody; Protein-glutamine N-methyltransferase PrmC antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) prmC protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.

Gene References into Functions

Results describe the crystal structure of the complex between E. coli RF1 and PrmC bound to the methyl donor product AdoHCy. PMID: 16364916
HemK is a Protein-glutamine N- methyltransferase PMID: 11805295

Protein Families

Protein N5-glutamine methyltransferase family, PrmC subfamily

Database Links

KEGG: ecj:JW1203

STRING: 316385.ECDH10B_1265

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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prmC Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background