PRF5 Antibody

Code CSB-PA658517XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PRF5 Polyclonal antibody
Uniprot No.
Target Names
PRF5
Alternative Names
PRF5 antibody; PFN4 antibody; PRF4 antibody; PRO4 antibody; At2g19770 antibody; F6F22.20 antibody; Profilin-5 antibody; AtPROF4 antibody; AthPRF4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PRF5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Binds to actin monomers and regulates the organization of the actin cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. At low concentrations, associates with the poly-proline motif of formins to enhance actin filament elongation rate. Acts redundantly with PRF4 to regulate apical actin polymerization at the tip of pollen tube and control polarized pollen tube growth. Functions probably by favoring formin-mediated actin polymerization at pollen tube tips.
Gene References into Functions
  1. PRF4 and PRF5 regulate actin polymerization in pollen grains and tubes. PMID: 26433093
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Profilin family
Tissue Specificity
Specifically expressed in mature pollen grains. Expressed in germinating pollen grains. Expressed in growing pollen tubes (at protein level).
Database Links

KEGG: ath:AT2G19770

STRING: 3702.AT2G19770.1

UniGene: At.39795

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