PRP16 Antibody

Code CSB-PA006880XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PRP16 Polyclonal antibody
Uniprot No.
Target Names
PRP16
Alternative Names
PRP16 antibody; YKR086W antibody; YKR406 antibody; Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 antibody; EC 3.6.4.13 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PRP16 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Influences the fidelity of branchpoint recognition in yeast splicing. This is RNA-dependent ATPase which is essential for viability. It may mediate one of the many ATP-requiring steps of spliceosome assembly and that accuracy of branchpoint recognition may be coupled to ATP binding and/or hydrolysis.
Gene References into Functions
  1. In the process of pre-mRNA splicing, Cwc25 binds tightly to the spliceosome after the reaction and is then removed from the spliceosome, which normally requires Prp16 and ATP hydrolysis, to allow the occurrence of the second reaction. PMID: 28057857
  2. At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites. PMID: 26919433
  3. Results indicate that the prp16-302 mutation in Prp16 stabilizes Cwc2 interactions with U6 snRNA and destabilizes Cwc2 interactions with pre-mRNA, indicating antagonistic functions of Cwc2 and Prp16. PMID: 24848011
  4. NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome. PMID: 23166295
  5. Prp16 remodels an interaction involving Isy1 in the transition between two steps of splicing PMID: 16103217

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Subcellular Location
Nucleus. Note=Binds to the spliceosome.
Protein Families
DEAD box helicase family, DEAH subfamily, PRP16 sub-subfamily
Database Links

KEGG: sce:YKR086W

STRING: 4932.YKR086W

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