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Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (PRP16), partial

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (PRP16), partial

Code	CSB-YP006880SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP006880SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP006880SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP006880SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP006880SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRP16

Uniprot No.

P15938

Alternative Names

PRP16; YKR086W; YKR406; Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16; EC 3.6.4.13

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Influences the fidelity of branchpoint recognition in yeast splicing. This is RNA-dependent ATPase which is essential for viability. It may mediate one of the many ATP-requiring steps of spliceosome assembly and that accuracy of branchpoint recognition may be coupled to ATP binding and/or hydrolysis.

Gene References into Functions

In the process of pre-mRNA splicing, Cwc25 binds tightly to the spliceosome after the reaction and is then removed from the spliceosome, which normally requires Prp16 and ATP hydrolysis, to allow the occurrence of the second reaction. PMID: 28057857
At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites. PMID: 26919433
Results indicate that the prp16-302 mutation in Prp16 stabilizes Cwc2 interactions with U6 snRNA and destabilizes Cwc2 interactions with pre-mRNA, indicating antagonistic functions of Cwc2 and Prp16. PMID: 24848011
NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome. PMID: 23166295
Prp16 remodels an interaction involving Isy1 in the transition between two steps of splicing PMID: 16103217

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Subcellular Location

Nucleus. Note=Binds to the spliceosome.

Protein Families

DEAD box helicase family, DEAH subfamily, PRP16 sub-subfamily

Database Links

KEGG: sce:YKR086W

STRING: 4932.YKR086W

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