Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPT2A Polyclonal antibody

Uniprot No.

Q9SZD4

Target Names

RPT2A

Alternative Names

RPT2A antibody; HLR antibody; At4g29040 antibody; F19B15.70 antibody; F25O24.6 antibody; 26S proteasome regulatory subunit 4 homolog A antibody; 26S proteasome AAA-ATPase subunit RPT2a antibody; 26S proteasome subunit 4 homolog A antibody; Protein HALTED ROOT antibody; Regulatory particle triple-A ATPase subunit 2a antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RPT2A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (Probable). Interacts with transit peptides of proteins targeted to the chloroplast, and may be involved in the degradation of unimported plastid protein precursors. Is required for the maintenance of postembryonic root and shoot meristems. Has a specific role in the regulation of organs size. Acts redundantly with RPT2B in the regulation of gametogenesis. With RPT2B plays a critical role in 26S proteasome assembly. Acts as an upstream signaling component for inducing both defense and morphological phenotypes in the constitutive active uni-1D mutant. Acts as negative regulator of endoreduplication in trichome cells. May function after the completion of the third endoreduplication step (8C to 16C) mediated by RHL1. Acts as negative regulator of transcriptional gene silencing (TGS) at specific endogenous genes through DNA methylation. Promotes post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS. Involved in tolerance to zinc deficiency, possibly through alleviation of oxidative stresses or processing of poly-ubiquitinated proteins. Required for resistance to the fungal pathogen Golovinomyces cichoracearum.

Gene References into Functions

Both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant. PMID: 22615900
Loss of RPT2a destabilizes the 26S proteasome. PMID: 22158466
RPT2a and RPT2b contribute differently to the proteasome activity required for root apical meristem growth and gametophyte development. PMID: 21784786
RPT2a and RPT2b, the subunits of the 26S proteasome AAA-ATPase, are UNI-interacting proteins. uni-1D-induced morphological phenotypes during early vegetative stage were suppressed by loss of function of RPT2a, but not RPT2b. PMID: 21791544
Data suggest that RPT2a functions to negatively regulate endocycle progression following completion of the third endoreduplication step mediated by RHL1. PMID: 20195883
RPT2a has a specific role in the regulation of leaf organ size that involves control of endoreduplication and cell size, rather than alteration of leaf cell number. PMID: 19500299

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Subcellular Location

Cytoplasm, P-body. Nucleus.

Protein Families

AAA ATPase family

Tissue Specificity

Preferentially expressed in the root and shoot apical meristem.

Database Links

KEGG: ath:AT4G29040

STRING: 3702.AT4G29040.1

UniGene: At.24596

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RPT2A Antibody

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