RPT5A Antibody

Code CSB-PA865864XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPT5A Polyclonal antibody
Uniprot No.
Target Names
RPT5A
Alternative Names
RPT5A antibody; ATS6A.2 antibody; TBP1 antibody; At3g05530 antibody; F22F7.1 antibody; 26S proteasome regulatory subunit 6A homolog A antibody; 26S proteasome AAA-ATPase subunit RPT5a antibody; Proteasome 26S subunit 6A homolog A antibody; Regulatory particle triple-A ATPase subunit 5a antibody; Tat-binding protein 1 homolog A antibody; TBP-1 homolog A antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPT5A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (Probable). Interacts with transit peptides of proteins targeted to the chloroplast, and may be involved in the degradation of unimported plastid protein precursors. Plays a essential role in the gametophyte development. Involved in tolerance to zinc deficiency, possibly through alleviation of oxidative stresses or processing of poly-ubiquitinated proteins.
Gene References into Functions
  1. These results suggest that the ahg12 mutation, a missense mutation in RPT5a, led to changes in the substrate preference of the 26S proteasome. PMID: 27139926
  2. RPT2a and RPT5a are involved in Zn deficiency-tolerance, possibly through alleviation of oxidative stresses and/or processing of poly-ubiquitinated proteins. PMID: 21389614
  3. The accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing PMID: 20682047
  4. RPT5 subunits are essential for both male and female gametophyte development. PMID: 19223514
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
AAA ATPase family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT3G05530

STRING: 3702.AT3G05530.1

UniGene: At.11513

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