pspA Antibody

Datasheet

Code	CSB-PA360290XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) pspA Polyclonal antibody

Uniprot No.

P0AFM6

Target Names

pspA

Alternative Names

pspA antibody; b1304 antibody; JW1297 antibody; Phage shock protein A antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) pspA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force.

Gene References into Functions

Structure and function of Escherichia coli PspA and Synechocystis Vipp1 N-terminal peptides in the membrane stress sensing and mitigation have been described. PMID: 27806910
Escherichia coli PspA has an enhanced sensitivity for curvature elastic (SCE) stress and a higher affinity for the membrane than Synechocystis Vipp1. PMID: 26330516
The discrete spatial distribution and dynamics of PspA effector in lateral membrane regions depend on MreB and RodZ. PMID: 25118250
Multiple signals are needed to induce the Psp response. PMID: 21259006
These results indicate that oligomers of PspA bind to membrane phospholipids and suppress proton leakage. PMID: 17725563
PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface. PMID: 18789328
PspA and PspG are localized to the cell poles and traffic between both poles. PMID: 19555453

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Subcellular Location

Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes at both cell poles and along the length of the cell.

Protein Families

PspA/IM30 family

Database Links

KEGG: ecj:JW1297

STRING: 316385.ECDH10B_1421

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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pspA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background