ptsI Antibody

Code CSB-PA362529XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ptsI Polyclonal antibody
Uniprot No.
Target Names
ptsI
Alternative Names
ptsI antibody; b2416 antibody; JW2409 antibody; Phosphoenolpyruvate-protein phosphotransferase antibody; EC 2.7.3.9 antibody; Phosphotransferase system antibody; enzyme I antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) ptsI protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). Can also use (Z)-3-fluoro-PEP (ZFPEP), (Z)-3-methyl-PEP (ZMePEP), (Z)-3-chloro-PEP (ZClPEP) and (E)-3-chloro-PEP (EClPEP) as alternative phosphoryl donors.
Gene References into Functions
  1. Study elucidated the structure of the NPr:EIN Ntr complex using a hybrid approach. The structure has a similar fold to the homologous HPr:EIN sugar complex, but differs in surface potential, which explains the difference in specificity between the two evolutionarily related enzyme pairs. PMID: 27839951
  2. Isolated a stable recombinant C-terminal domain of Enzyme I (EIC) of Escherichia coli and characterized its interaction with the N-terminal domain of Enzyme I (EIN) and also with various ligands. PMID: 22936614
  3. Subcellular distribution depends on growth conditions PMID: 15557553
  4. the phosphotransfer rate increases approximately 25-fold when HPr is added to a mixture of EI-N, EI-C, and PEP(Mg(2+)) PMID: 16547354
  5. EI and EI-H189Q each comprise a multiplicity of conformers and progressively fewer conformers as they dimerize and bind Mg(2+) and finally PEP PMID: 16547355
  6. crystal structure reveals a dimeric protein where each subunit comprises three domains PMID: 17053069

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Subcellular Location
Cytoplasm.
Protein Families
PEP-utilizing enzyme family
Database Links
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