PTAC12 Antibody

Datasheet

Code	CSB-PA931347XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PTAC12 Polyclonal antibody

Uniprot No.

F4IHY7

Target Names

PTAC12

Alternative Names

PTAC12 antibody; HMR antibody; PAP5 antibody; TAC12 antibody; At2g34640 antibody; T31E10.2 antibody; Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 antibody; chloroplastic antibody; pTAC12 antibody; Plastid-encoded RNA polymerase-associated protein 5 antibody; PEP-associated protein 5 antibody; Protein HEMERA antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PTAC12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in plastid gene expression. Required in the nucleus for the initiation of photomorphogenesis mediated by phytochromes (PHYs) (e.g. PHYA and PHYB) by mediating PHYs localization to photobodies, especially in response to red and far-red light, and implicating phytochrome nuclear bodies as sites of proteolysis for PHYs and PIFs proteins (e.g. PIF1 and PIF3). Acts downstream of PHYs and upstream of DET1.

Gene References into Functions

Data suggest a retrograde protein translocation mechanism in which HEMERA/pTAC12 (HMR) is targeted first to plastids, processed to the mature form, and then relocated to the nucleus. PMID: 28232584
Arabidopsis pTAC14 is essential for proper chloroplast development, most likely by affecting plastid-encoded bacterial-type RNA polymerase (PEP) activity and regulating PEP-dependent plastid gene transcription in Arabidopsis together with pTAC12. PMID: 22010110
phytochromes directly interact with HMR to promote HMR protein accumulation in the light PMID: 22895253
HEMERA (previously identified as pTAC12) has a function in the nucleus, where it acts specifically in phytochrome signaling, is predicted to be similar to the multiubiquitin-binding protein, RAD23, and can partially rescue yeast rad23mutants. PMID: 20603003

Subcellular Location

Plastid, chloroplast. Nucleus.

Tissue Specificity

Mostly expressed in cotyledons, leaves, stems and flowers, but barely in roots.

Database Links

KEGG: ath:AT2G34640

STRING: 3702.AT2G34640.1

UniGene: At.37777

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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