NPF6.3 Antibody

Code CSB-PA313122XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NPF6.3 Polyclonal antibody
Uniprot No.
Target Names
NPF6.3
Alternative Names
NPF6.3 antibody; CHL1 antibody; NRT1 antibody; NRT1.1 antibody; At1g12110 antibody; F12F1.1 antibody; T28K15_13 antibody; Protein NRT1/ PTR FAMILY 6.3 antibody; AtNPF6.3 antibody; Nitrate transporter 1.1 antibody; AtNRT1 antibody; Nitrate/chlorate transporter antibody; Protein CHLORINA 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NPF6.3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation. Recognizes specifically nitrate and chlorate, but not nitrite, alanine, sulfate, phosphate or the di-peptide Ala-Ala.
Gene References into Functions
  1. CPSF30-L mediates nitrate signaling in part by regulating NRT1.1 expression. PMID: 28850721
  2. NRT1.1-mediated nitrate uptake plays a crucial role in plant proton tolerance by alkalifying the rhizosphere. PMID: 26864608
  3. Results show that although NO3(-) stimulates NRT1.1/NPF6.3 transporter/sensor transcription and probably mRNA stability both in primary root tissues and in lateral root primordia (LRPs), it acts differentially on protein accumulation, depending on the tissues considered with stimulation in cortex and epidermis of the primary root. PMID: 27543115
  4. Report activity/regulation of nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters using fluorescent sensors PMID: 24623305
  5. NRT1.1 may regulate the uptake of cadmium and other cations by a common mechanism PMID: 25106820
  6. gene expression in response to nitrate treatment of the AFB3 auxin receptor and its target, the NAC4 transcription factor, depends on the nitrate transport function of NRT1.1 PMID: 24642706
  7. NRT1.1 allows preferential root colonization of nitrate-rich patches by both preventing root growth in response to low nitrate, through modulation of auxin traffic, and stimulating root growth in response to high nitrate. PMID: 23731054
  8. Data indicate that nitrate uptake through AtNRT1.1 conforms to a single low-affinity transport system that makes virtually no contribution to high-affinity nitrate uptake. PMID: 24089435
  9. crystal structure of unphosphorylated NRT1.1, which reveals an unexpected homodimer in the inward-facing conformation; results establish a phosphorylation-controlled dimerization switch that allows NRT1.1 to uptake nitrate with two distinct affinity modes PMID: 24572362
  10. the apo and nitrate-bound crystal structures of Arabidopsis thaliana NRT1.1, which together with in vitro binding and transport data identify a key role for His 356 in nitrate binding PMID: 24572366
  11. NRT1.1 mutants show enhanced tolerance to ammonium and/or low pH conditions in the absence of nitrate. PMID: 21448006
  12. NRT1.1 acts as a NO3- sensor and controls several NO3- signalling responses.[review] PMID: 21239382
  13. NRT1.1 mutants showed enhanced tolerance to concentrated ammonium in the absence of nitrate. PMID: 21052766
  14. NRT1.1 represses lateral root growth at low nitrate availability by promoting basipetal auxin transport out of these roots. This defines a mechanism connecting nutrient and hormone signaling during organ development. PMID: 20627075
  15. NRT1.1, which is localized at the forefront of soil exploration by the roots, is a key component of the NO(3)(-)-sensing system PMID: 17148611
  16. These results strongly support the model that NRT1.1 acts as a nitrate regulator or sensor in Arabidopsis. PMID: 19633234
  17. CHL1 uses dual-affinity binding and a phosphorylation switch to sense a wide range of nitrate concentrations in the soil, thereby functioning as an ion sensor in higher plants. PMID: 19766570

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
PTR2/POT transporter (TC 2.A.17) family
Tissue Specificity
Expressed in the stele in lateral root primordia before emergence and in the tip of primary and emerged lateral roots. Detected in emerging and immature leaves, guard cells, flower buds, style, stigma, anthers and pollen grains. Not detected in the shoot
Database Links

KEGG: ath:AT1G12110

STRING: 3702.AT1G12110.1

UniGene: At.19624

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