Product Details

Purity

>85% (SDS-PAGE)

Target Names

NPF6.3

Uniprot No.

Q05085

Alternative Names

NPF6.3; CHL1; NRT1; NRT1.1; At1g12110; F12F1.1; T28K15_13; Protein NRT1/ PTR FAMILY 6.3; AtNPF6.3; Nitrate transporter 1.1; AtNRT1; Nitrate/chlorate transporter; Protein CHLORINA 1

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation. Recognizes specifically nitrate and chlorate, but not nitrite, alanine, sulfate, phosphate or the di-peptide Ala-Ala.

Gene References into Functions

CPSF30-L mediates nitrate signaling in part by regulating NRT1.1 expression. PMID: 28850721
NRT1.1-mediated nitrate uptake plays a crucial role in plant proton tolerance by alkalifying the rhizosphere. PMID: 26864608
Results show that although NO3(-) stimulates NRT1.1/NPF6.3 transporter/sensor transcription and probably mRNA stability both in primary root tissues and in lateral root primordia (LRPs), it acts differentially on protein accumulation, depending on the tissues considered with stimulation in cortex and epidermis of the primary root. PMID: 27543115
Report activity/regulation of nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters using fluorescent sensors PMID: 24623305
NRT1.1 may regulate the uptake of cadmium and other cations by a common mechanism PMID: 25106820
gene expression in response to nitrate treatment of the AFB3 auxin receptor and its target, the NAC4 transcription factor, depends on the nitrate transport function of NRT1.1 PMID: 24642706
NRT1.1 allows preferential root colonization of nitrate-rich patches by both preventing root growth in response to low nitrate, through modulation of auxin traffic, and stimulating root growth in response to high nitrate. PMID: 23731054
Data indicate that nitrate uptake through AtNRT1.1 conforms to a single low-affinity transport system that makes virtually no contribution to high-affinity nitrate uptake. PMID: 24089435
crystal structure of unphosphorylated NRT1.1, which reveals an unexpected homodimer in the inward-facing conformation; results establish a phosphorylation-controlled dimerization switch that allows NRT1.1 to uptake nitrate with two distinct affinity modes PMID: 24572362
the apo and nitrate-bound crystal structures of Arabidopsis thaliana NRT1.1, which together with in vitro binding and transport data identify a key role for His 356 in nitrate binding PMID: 24572366
NRT1.1 mutants show enhanced tolerance to ammonium and/or low pH conditions in the absence of nitrate. PMID: 21448006
NRT1.1 acts as a NO3- sensor and controls several NO3- signalling responses.[review] PMID: 21239382
NRT1.1 mutants showed enhanced tolerance to concentrated ammonium in the absence of nitrate. PMID: 21052766
NRT1.1 represses lateral root growth at low nitrate availability by promoting basipetal auxin transport out of these roots. This defines a mechanism connecting nutrient and hormone signaling during organ development. PMID: 20627075
NRT1.1, which is localized at the forefront of soil exploration by the roots, is a key component of the NO(3)(-)-sensing system PMID: 17148611
These results strongly support the model that NRT1.1 acts as a nitrate regulator or sensor in Arabidopsis. PMID: 19633234
CHL1 uses dual-affinity binding and a phosphorylation switch to sense a wide range of nitrate concentrations in the soil, thereby functioning as an ion sensor in higher plants. PMID: 19766570

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

PTR2/POT transporter (TC 2.A.17) family

Tissue Specificity

Expressed in the stele in lateral root primordia before emergence and in the tip of primary and emerged lateral roots. Detected in emerging and immature leaves, guard cells, flower buds, style, stigma, anthers and pollen grains. Not detected in the shoot

Database Links

KEGG: ath:AT1G12110

STRING: 3702.AT1G12110.1

UniGene: At.19624

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Recombinant Arabidopsis thaliana Nitrate transporter 1.1 (NRT1.1), partial

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