Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Patronin Polyclonal antibody

Uniprot No.

A1ZAU8

Target Names

Patronin

Alternative Names

Patronin antibody; l(2)k07433 antibody; ssp4 antibody; CG33130 antibody; Patronin antibody; Short spindle protein 4 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Patronin protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in mitotic spindle assembly. Regulates microtubule (MT) severing. Antagonizes the activity of the kinesin-13 depolymerase Klp10A thereby switching off the depolymerization of the MTs at their pole-associated minus ends, which turns off poleward flux and induces anaphase B spindle elongation. Involved in asymmetric cell division of sensory organ precursor (SOP) cells by playing a role in the asymmetric localization of Sara-expressing endosomes to the pIIa daughter cell but not to the pIIb cell. Klp98A targets Sara-expressing endosomes to the central spindle which is symmetrically arranged in early cell division. During late cytokinesis, central spindle asymmetry is generated by enrichment of Patronin on the pIIb side which protects microtubules from depolymerization by Klp10A while unprotected microtubules on the pIIa side are disassembled by Klp10A, leading to the asymmetric delivery of Sara-expressing endosomes to the pIIa daughter cell.

Gene References into Functions

These results show that members of the CAMSAP/Patronin family all localize to and protect minus-ends but have evolved distinct effects on microtubule dynamics. PMID: 24706919
A recent study provides the first definitive identification of a minus-end-specific capping protein, termed Patronin, which protects the microtubule arrays of both mitotic and interphase cells. PMID: 21256440
Ssp4, renamed Patronin, protects microtubule minus ends against depolymerization by Kinesin-13; in its absence, microtubules release from their nucleating sites and treadmill through the cytoplasm, a result of unhindered minus end depolymerization. PMID: 20946984

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to centrosomes in prophase, to the midbody during cytokinesis, diffusely throughout the metaphase spindle and to punctae in interphase that often overlap with MTs. Colocalizes with Sas-4 and SAK at the microtubule organizing center.

Protein Families

CAMSAP1 family

Database Links

KEGG: dme:Dmel_CG33130

UniGene: Dm.20641

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Patronin Antibody

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