ptsN Antibody

Code CSB-PA301715XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ptsN Polyclonal antibody
Uniprot No.
Target Names
ptsN
Alternative Names
ptsN antibody; rpoP antibody; yhbI antibody; b3204 antibody; JW3171 antibody; Nitrogen regulatory protein antibody; Enzyme IIA-NTR antibody; PTS system EIIA component antibody; Phosphotransferase enzyme IIA component antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
A synthesized peptide derived from Escherichia coli (strain K12) ptsN protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, Dot blot (guaranteed specific to the immunizing peptide)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;
Quality Guarantee
1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Seems to have a role in regulating nitrogen assimilation.
Gene References into Functions
  1. Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation. PMID: 23517463
  2. Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG. PMID: 21531803
  3. PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level. PMID: 21143318
  4. The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy. PMID: 15741344
  5. the dephospho-form of enzyme IIA(Ntr) is required for derepression of the ilvBN operon encoding acetohydroxy acid synthase I catalysing the first step common to the biosynthesis of the branched-chain amino acids. PMID: 16164569
  6. overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E). PMID: 18253509
  7. EIIANtr is readily phosphorylated at the histidine 73. PMID: 18625021

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Subcellular Location
Cytoplasm.
Database Links
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