ptsN Antibody

Datasheet

Code	CSB-PA301715XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ptsN Polyclonal antibody

Uniprot No.

P69829

Target Names

ptsN

Alternative Names

ptsN antibody; rpoP antibody; yhbI antibody; b3204 antibody; JW3171 antibody; Nitrogen regulatory protein antibody; Enzyme IIA-NTR antibody; PTS system EIIA component antibody; Phosphotransferase enzyme IIA component antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ptsN protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Seems to have a role in regulating nitrogen assimilation.

Gene References into Functions

Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation. PMID: 23517463
Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG. PMID: 21531803
PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level. PMID: 21143318
The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy. PMID: 15741344
the dephospho-form of enzyme IIA(Ntr) is required for derepression of the ilvBN operon encoding acetohydroxy acid synthase I catalysing the first step common to the biosynthesis of the branched-chain amino acids. PMID: 16164569
overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E). PMID: 18253509
EIIANtr is readily phosphorylated at the histidine 73. PMID: 18625021

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Subcellular Location

Cytoplasm.

Database Links

KEGG: ecj:JW3171

STRING: 316385.ECDH10B_3378

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ptsN Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background