bglF Antibody

Datasheet

Code	CSB-PA357471XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) bglF Polyclonal antibody

Uniprot No.

P08722

Target Names

bglF

Alternative Names

bglF antibody; bglC antibody; bglS antibody; b3722 antibody; JW3700 antibody; PTS system beta-glucoside-specific EIIBCA component antibody; EIIBCA-Bgl antibody; EII-Bgl) [Includes: Beta-glucoside-specific phosphotransferase enzyme IIB component antibody; EC 2.7.1.- antibody; PTS system beta-glucoside-specific EIIB component); Beta-glucoside permease IIC component antibody; PTS system beta-glucoside-specific EIIC component); Beta-glucoside-specific phosphotransferase enzyme IIA component antibody; PTS system beta-glucoside-specific EIIA component)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) bglF protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in beta-glucoside transport.; Acts as both a kinase and a phosphatase on BglG.

Gene References into Functions

analysis of the Escherichia coli beta-glucoside transporter BglF membrane topology PMID: 15755739
This study identified distinct sites in BglF that are involved in regulating phosphate flow via the common active-site residue in response to environmental cues. PMID: 17905978
The results demonstrate that the big cytoplasmic loop participates in creating the sugar pathway and explain the observed coupling between translocation of phosphotransferase system sugars from the periplasm to the cytoplasm and their phosphorylation. PMID: 19251853

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW3700

STRING: 316385.ECDH10B_3909

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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bglF Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background