nagE Antibody

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Code	CSB-PA357643XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) nagE Polyclonal antibody

Uniprot No.

P09323

Target Names

nagE

Alternative Names

nagE antibody; pstN antibody; b0679 antibody; JW0665 antibody; PTS system N-acetylglucosamine-specific EIICBA component antibody; EIICBA-Nag antibody; EII-Nag) [Includes: N-acetylglucosamine permease IIC component antibody; PTS system N-acetylglucosamine-specific EIIC component); N-acetylglucosamine-specific phosphotransferase enzyme IIB component antibody; EC 2.7.1.193 antibody; PTS system N-acetylglucosamine-specific EIIB component); N-acetylglucosamine-specific phosphotransferase enzyme IIA component antibody; PTS system N-acetylglucosamine-specific EIIA component)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) nagE protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylglucosamine transport. It can also transport and phosphorylate the antibiotic streptozotocin. Could play a significant role in the recycling of peptidoglycan.

Gene References into Functions

analysis of conformational changes in the membrane-embedded IIC(mtl)-domain of the mannitol permease from Escherichia coli PMID: 16093245
findings show that the nagE-encoded PTS transporter makes an important contribution to the recycling of peptidoglycan. PMID: 19617367

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW0665

STRING: 316385.ECDH10B_0744

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nagE Antibody

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Target Background