PUB4 Antibody

Code CSB-PA515768XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PUB4 Polyclonal antibody
Uniprot No.
Target Names
PUB4
Alternative Names
PUB4 antibody; At2g23140 antibody; T20D16.23U-box domain-containing protein 4 antibody; EC 2.3.2.27 antibody; Plant U-box protein 4 antibody; RING-type E3 ubiquitin transferase PUB4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PUB4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Functions as an E3 ubiquitin ligase.
Gene References into Functions
  1. PUB4 role in the cytokinin signaling networks regulating developmental and physiological processes.PUB4 interaction with XLG1, XLG2 and XlG3 proteins. PMID: 27986866
  2. PLANT U-BOX 4 (PUB4), is a key regulatory component of root meristem maintenance that functions downstream of an exogenous synthetic CLV3 peptide. PMID: 25898239
  3. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for ubiquitination and selective degradation of chloroplasts damaged by reactive oxygen species. PMID: 26494759
  4. PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/endodermis initial daughters. PMID: 25605779
  5. AtPUB4-mediated pathway acts in parallel with the brassinosteroid pathway in controlling developmental fates of the tapetal cells to ensure male fertility. [PUB4] PMID: 23398263

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Database Links

KEGG: ath:AT2G23140

STRING: 3702.AT2G23140.1

UniGene: At.39339

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