puf-5 Antibody

Code CSB-PA210098XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans puf-5 Polyclonal antibody
Uniprot No.
Target Names
puf-5
Alternative Names
puf-5 antibody; F54C9.8 antibody; Pumilio domain-containing protein 5 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans puf-5 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
RNA-binding protein that binds to the consensus sequence 5'-CUCUGUAUCUUGU-3' in mRNA 3'-UTRs and modulates mRNA expression and stability. Functions redundantly with puf-6 and puf-7 in oocyte formation and organization, early embryonic cell divisions, and repression of expression of glp-1 and other maternal mRNAs in late oogenesis.
Gene References into Functions
  1. the PUF domain protein PUF-5 and two nearly identical PUF proteins PUF-6 and PUF-7 function during a specific period of oocyte differentiation to repress glp-1 and other maternal mRNAs PMID: 17234175
  2. PUF-5 and PUF-6 recognize similar RNA sequences, and these are distinct from those seen by members of the FBF and PUF-8/-9 groups; PUF-5/-6/-7 regulate overlapping (or identical) sets of target mRNAs. PMID: 18579869
Subcellular Location
Cytoplasm. Cytoplasm, P-body.
Tissue Specificity
Detected in differentiating oocytes with highest levels observed in developing ooctyes in the distal portion of the proximal gonad.
Database Links

KEGG: cel:CELE_F54C9.8

STRING: 6239.F54C9.8

UniGene: Cel.23039

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