putP Antibody

Datasheet

Code	CSB-PA357030XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) putP Polyclonal antibody

Uniprot No.

P07117

Target Names

putP

Alternative Names

putP antibody; b1015 antibody; JW1001 antibody; Sodium/proline symporter antibody; Proline permease antibody; Propionate transporter antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) putP protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate.

Gene References into Functions

the results demonstrate that TM6' plays a central role in substrate binding and release on the inner side of the membrane also in PutP and extend the knowledge on functionally relevant amino acids in transporters with a LeuT-type structural fold. PMID: 27793991
Remarkably,as proposed computationally and discovered here experimentally, residues Y140, W244, and Y248 of transmembrane segments 4 and 7 are particularly important for PutP function and are suggested to participate in proline binding and/or gating. PMID: 21130773
backbone models of PutP exhibiting a pronounced kink near residue T341, which is involved in substrate binding, were obtained. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity. PMID: 19134477
Results describe the function of transmembrane domain IX in the Na+/proline transporter PutP. PMID: 18692508

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Sodium:solute symporter (SSF) (TC 2.A.21) family

Database Links

KEGG: ecj:JW1001

STRING: 316385.ECDH10B_1087

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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putP Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background