PYL8 Antibody

Code CSB-PA880729XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PYL8 Polyclonal antibody
Uniprot No.
Target Names
PYL8
Alternative Names
PYL8 antibody; ABIP1 antibody; RCAR3 antibody; At5g53160 antibody; MFH8.10 antibody; Abscisic acid receptor PYL8 antibody; ABI1-binding protein 1 antibody; PYR1-like protein 8 antibody; Regulatory components of ABA receptor 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PYL8 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) in an ABA-independent manner but more efficiently when activated by ABA. Confers enhanced sensitivity to ABA. Can be activated by both (-)-ABA and (+)-ABA. Mediates crosstalk between ABA and auxin signaling to regulate lateral root growth. Required for lateral root growth suppression by ABA. In response to auxin, promotes lateral root growth by enhancing MYB77-dependent transcription of the auxin-responsive gene IAA19. Enhances the abilities of MYB44 and MYB73 to activate IAA19 gene.
Gene References into Functions
  1. Results confirm that PYL8 plays an important role in regulating lateral root growth of aeabidopsis. PMID: 27256015
  2. RIFP1 plays a negative role in the RCAR3-mediated abscissic acid signalling pathway. PMID: 26386272
  3. Overexpression of PYL8 resulted in significantly reduced seed germination and cotyledon greening under high glucose conditions, while RNAi transgenic lines were more insensitive to glucose stress. PMID: 25983319
  4. The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes. PMID: 24894996
Subcellular Location
Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane.
Protein Families
PYR/PYL/RCAR abscisic acid intracellular receptor family
Database Links

KEGG: ath:AT5G53160

STRING: 3702.AT5G53160.2

UniGene: At.24684

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