queF Antibody

Code CSB-PA676103XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) queF Polyclonal antibody
Uniprot No.
Target Names
queF
Alternative Names
queF antibody; yqcD antibody; b2794 antibody; JW2765 antibody; NADPH-dependent 7-cyano-7-deazaguanine reductase antibody; EC 1.7.1.13 antibody; 7-cyano-7-carbaguanine reductase antibody; NADPH-dependent nitrile oxidoreductase antibody; PreQ(0) reductase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) queF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a late step in the queuosine pathway. Is highly specific for its natural substrate preQ0, since it cannot use various aliphatic, aromatic, benzylic and heterocyclic nitriles, such as acetonitrile, benzonitrile, benzylcyanide and 2-cyanopyrrole, although it can reduce the substrate analog 5-cyanopyrrolo[2,3-d]pyrimidin-4-one with lesser efficiency.
Gene References into Functions
  1. A model of QueF substrate recognition and a catalytic pathway for the enzyme are proposed based on these data. PMID: 27754868
  2. The expression of NP-417272 after cloning in E coli BL21 cells was studied, and optimal conditions for its activity toward its natural substrate were determined. PMID: 23410922
Subcellular Location
Cytoplasm.
Protein Families
GTP cyclohydrolase I family, QueF type 2 subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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