RPP13L4 Antibody

Datasheet

Code	CSB-PA653624XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPP13L4 Polyclonal antibody

Uniprot No.

Q38834

Target Names

RPP13L4

Alternative Names

RPP13L4 antibody; ZAR1 antibody; At3g50950 antibody; F18B3.230 antibody; Disease resistance RPP13-like protein 4 antibody; Disease resistance protein ZAR1 antibody; Protein HOPZ-ACTIVATED RESISTANCE 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RPP13L4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

CC-NB-LRR receptor-like protein required for recognition of pathogenic bacteria type III effectors (T3E) such as Pseudomonas syringae HopZ1a and HopF2a and Xanthomonas campestris pv. campestris (Xcc) XopAC/AvrAC; this recognition requires ZED1-related kinases (e.g. PBL2, ZRK3 and ZED1/ZRK5). Confers allele-specific recognition and virulence attenuation of HopZ1a. Immunity mediated by RPP13L4/ZAR1 is independent of several genes required by other resistance protein signaling pathways such as NDR1 and RAR1. Together with ZED1/ZRK5, involved in the regulation of the ambient temperature-sensitive intersection of growth and immune response in the absence of pathogens.

Gene References into Functions

The Arabidopsis ZAR1 NLR protein requires the ZRK3 kinase to recognize the Pseudomonas syringae type III effector (T3E) HopF2a. PMID: 28288096
ZAR1 role in high temperature-induced autoimmunity PMID: 28499073
This detailed analysis of the AtZAR1-AtZED1 protein complex provides a better understanding of the immune signaling hub controlled by AtZAR1. PMID: 28652264
The disruption of ZAR1 and AGB1 results in short basal cell and an apical cell with basal cell fate PMID: 27014878
Arabidopsis resistance protein ZAR1 confers allele-specific recognition and virulence attenuation of the Pseudomonas syringae T3SE protein HopZ1a. [ZAR1] PMID: 20368970

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Subcellular Location

Cell membrane. Nucleus.

Protein Families

Disease resistance NB-LRR family, RPP13 subfamily

Database Links

KEGG: ath:AT3G50950

STRING: 3702.AT3G50950.1

UniGene: At.3283

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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RPP13L4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background