ARAC11 Antibody

Code CSB-PA310507XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARAC11 Polyclonal antibody
Uniprot No.
Target Names
ARAC11
Alternative Names
ARAC11 antibody; RAC11 antibody; ROP1 antibody; At3g51300 antibody; F24M12.340 antibody; Rac-like GTP-binding protein ARAC11 antibody; GTPase protein ROP1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARAC11 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May be involved in cell polarity control during the actin-dependent tip growth of pollen tubes. May regulate callose synthase 1 (CALS1) activity through the interaction with UGT1.; Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation.
Gene References into Functions
  1. These findings provide a founding framework revealing auxin and ROP signaling of inner polar nuclear position. PMID: 29084900
  2. dominant negative form affects potato resistance to Phytophthora infestans; this is associated with increased NADPH oxidase-mediated H2O2 production and JA signaling PMID: 25547733
  3. ROP1 and ROP6 are required for F-actin bundle formation in the roots, which is required for P. indica-mediated growth promotion in Arabidopsis. PMID: 23118477
  4. Data propose that the balance between the lateral propagation and the global inhibition maintains an optimal apical ROP1 cap and generates the apical ROP1 activity oscillation required for efficient pollen-tube elongation. PMID: 20053639
  5. Correct positioning of the ROP patch depends on the cell length, low diffusion of active ROP, a gradient in auxin concentration, and ROP levels. PMID: 20016781
  6. RIP1 is involved in the positive feedback regulation of ROP1 localization to the plasma membrane, leading to the establishment of a polar site for pollen germination and pollen tube growth. PMID: 19825600
  7. ROP1 is a Rho-like GTPase from plants .Our report demonstrates the oscillation of Rho GTPase signaling, which may be a common mechanism underlying the oscillation of actin-dependent processes such as polar growth, cell movement, and chemotaxis. PMID: 16148045
  8. These results provide strong evidence that RopGEF1 activates ROP1 in control of polar growth in pollen tubes. PMID: 16415208
  9. in its GTP-bound configuration interacts with phragmoplastin-interacting protein PMID: 18621982
  10. A screen for mutations that enhance ROP1-overexpression-induced depolarization of pollen-tube growth identified REN1 (ROP1 enhancer 1), whose null mutations turn elongated pollen tubes into bulbous cells. PMID: 19108776

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein. Note=Associated with the membrane when activated.
Protein Families
Small GTPase superfamily, Rho family
Tissue Specificity
Exclusively expressed in mature pollen and pollen tubes.
Database Links

KEGG: ath:AT3G51300

STRING: 3702.AT3G51300.1

UniGene: At.28445

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