ARAC3 Antibody

Code CSB-PA656439XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARAC3 Polyclonal antibody
Uniprot No.
Target Names
ARAC3
Alternative Names
ARAC3 antibody; RAC1 antibody; ROP6 antibody; At4g35020 antibody; M4E13.80 antibody; Rac-like GTP-binding protein ARAC3 antibody; GTPase protein ROP6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARAC3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation. Involved in cell polarity control during the actin-dependent tip growth of root hairs, thus regulating root hair length and root hair initiation. SPK1-dependent activation is required for auxin-mediated inhibition of PIN2 internalization during gravitropic responses.
Gene References into Functions
  1. the equilibrium dissociation constants of AtROP66-178 with fluorescent guanine nucleotide analogue mantGDP and mantGTP were determined to be 0.0721 muM and 0.0422 muM, respectively, based on fluorescence polarization. PMID: 27789389
  2. Rho of Plants6 (AtROP6) integrates developmental and pathogen response signaling. PMID: 23319551
  3. Study demonstrates that auxin promotes interdigitated leaf epidermal pavement cell expansion by coordinately activating the antagonistic ROP2 and ROP6 pathways in an ABP1-dependent manner. PMID: 20887895
  4. ROP6 directly binds and activates microtubule-associated RIC1 to achieve microtubule ordering. PMID: 19818614
Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Protein Families
Small GTPase superfamily, Rho family
Tissue Specificity
Ubiquitous. Preferentially expressed at the tip of root hairs.
Database Links

KEGG: ath:AT4G35020

STRING: 3702.AT4G35020.1

UniGene: At.20553

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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