Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARAC4 Polyclonal antibody

Uniprot No.

Q38919

Target Names

ARAC4

Alternative Names

ARAC4 antibody; ROP2 antibody; At1g20090 antibody; T20H2.12 antibody; Rac-like GTP-binding protein ARAC4 antibody; GTPase protein ROP2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ARAC4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation. Involved in cell polarity control during the actin-dependent tip growth of root hairs, thus regulating root hair length and root hair initiation. May regulate a WAVE complex that activates the Arp2/3 complex.

Gene References into Functions

we reveal that the small GTPase Rho-related protein 2 (ROP2) transduces light-auxin signal to activate TOR by direct interaction, which, in turn, promotes transcription factors E2Fa,b for activating cell cycle genes in shoot apexes. Consistently, constitutively activated ROP2 plants stimulate TOR in the shoot apex and cause true leaf development even without light. PMID: 28223530
ROP2 is required for the formation of a fine endoplasmic reticulum structure in the subapical zone of growing root hairs. PMID: 27231102
RopGEF2 interacted with ROP7 and ROP2 and enhanced their intrinsic nucleotide exchange rates.Rop2 plays a role in the red lignt-induced stomatal opening. PMID: 28188273
Here, the authors report that the small GTPase ROP2, if activated by the phytohormone auxin, promotes activation of TOR, and thus translation reinitiation of uORF-containing mRNAs. PMID: 28246118
ABA inactivates ROP2 GTPase in guard cells. Active ROP2 suppresses the endocytotic internalization of plasma membrane, a process required for stomatal closure. PMID: 21883287
Reactive oxygen species formation and root hair growh is impaired when ROP2 is mutated. PMID: 17301029
ROP2 is a light-activated negative factor that attenuates the extent of light-induced changes in stomatal aperture. PMID: 18178769

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein.

Protein Families

Small GTPase superfamily, Rho family

Tissue Specificity

Ubiquitous.

Database Links

KEGG: ath:AT1G20090

STRING: 3702.AT1G20090.1

UniGene: At.23198

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ARAC4 Antibody

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