RAD2 Antibody

Code CSB-PA357076XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD2 Polyclonal antibody
Uniprot No.
Target Names
RAD2
Alternative Names
RAD2 antibody; YGR258C antibody; DNA repair protein RAD2 antibody; EC 3.1.-.- antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair.
Gene References into Functions
  1. Rad2 and rad27 overexpression increases genomic instability in yeast. Rad2 and Rad27 causes DNA breaks that are processed by different repair functions. PMID: 28704715
  2. THe data suggest that in contrast to related enzymes (FEN1 and EXO1), the Rad2 active site may be more accessible, which would create an exit route for DNA substrates without a free 5' end. PMID: 25120270
  3. Study shows that Rad2p interacts with PCNA through its proliferating cell nuclear antigen-binding domain (PCNA-BD) and the PCNA-BD of Rad2p plays a role in UV-induced mutagenesis. PMID: 24674623
  4. These observations suggest that RAD2 has a function in coordinating cell cycle regulation and damaged DNA repair. PMID: 20222011
Subcellular Location
Nucleus.
Protein Families
XPG/RAD2 endonuclease family, XPG subfamily
Database Links

KEGG: sce:YGR258C

STRING: 4932.YGR258C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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