RAD51 Antibody

Code CSB-PA019263XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAD51 Polyclonal antibody
Uniprot No.
Target Names
RAD51
Alternative Names
RAD51 antibody; At5g20850 antibody; F22D1.20 antibody; DNA repair protein RAD51 homolog 1 antibody; Rad51-like protein 1 antibody; AtRAD51 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAD51 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA. Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis.
Gene References into Functions
  1. Although the presence of RAD51 protein provides essential support for the action of DMC1, these results show no significant effect of the absence of RAD51 strand-exchange activity on meiotic crossing-over rates or patterns in different chromosomal regions or across the whole genome of Arabidopsis, strongly supporting the argument that DMC1 catalyses repair of all meiotic DNA breaks, not only non-sister cross-overs. PMID: 28797117
  2. RAD51 forms a protein complex with AtRAD51C-AtXRCC3 to facilitate RAD51 localization on chromosomes for meiotic recombination. PMID: 28562599
  3. The present study demonstrates for the first time the involvement of a host RAD51 protein in mungbean yellow mosaic India virus replication. PMID: 23575883
  4. Establishment and stabilisation of pairing of homologous centromeric and pericentromeric regions depends principally upon DMC1, while pairing and synapsis of euchromatic chromosome arms of homologues requires the presence of RAD51 PMID: 22532804
  5. AtRAD51 is required to ensure the fidelity of homologous recombination during interchromosomal exchanges. It may also be required to ensure the fidelity of homologous recombination in the interchromosomal exchanges initiated by AtDMC1. PMID: 22066484
  6. results show that loss of RAD51 caused hypersensitivity to the double-strand break-inducing agent bleomycin in P. patens but not Arabidopsis; data show importance of RAD51 for viability is independent of taxonomic position or complexity of an organism PMID: 17921313

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Subcellular Location
Nucleus.
Protein Families
RecA family, RAD51 subfamily
Tissue Specificity
Detected in various tissues. Higher expression in reproductive tissues than in vegetative tissues, with the highest expression level in young flower buds. At cellular level, is expressed at low levels in flower primordia, then at higher levels in young an
Database Links

KEGG: ath:AT5G20850

STRING: 3702.AT5G20850.1

UniGene: At.110

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