spn-A Antibody

Code CSB-PA635648XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) spn-A Polyclonal antibody
Uniprot No.
Target Names
spn-A
Alternative Names
spn-A antibody; DMR antibody; Rad51 antibody; CG7948 antibody; DNA repair protein Rad51 homolog antibody; Protein spindle-A antibody; RecA protein homolog antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) spn-A protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA.; Spindle genes are required for each of the symmetry-breaking steps that generate polarity during egg axis formation; oocyte positioning at the posterior of the cyst to generate the first AP polarity and inhibition of gurken (grk) signaling to the follicle cell layer to polarize first the AP axis and then DV axis. May have a role in female meiosis.
Gene References into Functions
  1. DmRad51/spnA null mutants are viable but oogenesis is disrupted by the activation of a meiotic recombination checkpoint. PMID: 14592983
  2. Ectopically expressed DmRad51 disrupts cell cycle regulation and induces apoptosis. PMID: 15257466
Subcellular Location
Nucleus.
Protein Families
RecA family, RAD51 subfamily
Tissue Specificity
Highly expressed in ovaries.
Database Links

KEGG: dme:Dmel_CG7948

STRING: 7227.FBpp0084955

UniGene: Dm.1913

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