Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Rad54l Polyclonal antibody

Uniprot No.

P70270

Target Names

Rad54l

Alternative Names

Rad54l antibody; Rad54 antibody; DNA repair and recombination protein RAD54-like antibody; EC 3.6.4.- antibody; RAD54 homolog antibody; mHR54 antibody; mRAD54 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Rad54l protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations.

Gene References into Functions

Our study highlights the importance of the cooperation between Rad54 and Mus81 for mediating DNA DSB repair and restraining chromosome missegregation. PMID: 26876210
Rad54 is not required for either transgene isotype switching or transgene hypermutation. PMID: 12218123
Rad54-mediated homologous recombination is not essential for somatic hypermutation. In addition, the finding that the ablation of RAD51 paralogues causes an increase in SHM argues against a direct involvement of HR in promoting SHM. PMID: 12548566
Rad54 has a role in telomere length maintenance PMID: 12897131
Rad54 cooperates with DNA Ligase IV to support cellular proliferation, dna repair, and prevent chromosome and single chromatid aberrations PMID: 15175260
Mus81-Eme1- and Rad54-mediated homologous recombination are involved in the same DNA replication-dependent interstrand crosslinks repair pathway PMID: 17036055
These results reveal a differential interaction of the ATM-mediated DNA damage response and Rad54 paralog-mediated homologous recombination depending on the DNA damaging agent that initiates the response. PMID: 19061978

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Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family

Tissue Specificity

Hardly detectable in most tissues. Dramatically increased in thymus, spleen and testis.

Database Links

KEGG: mmu:19366

STRING: 10090.ENSMUSP00000099765

UniGene: Mm.3655

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Rad54l Antibody

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