rag1 Antibody

Code CSB-PA845862XA01GIH
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Product Details

Full Product Name
Rabbit anti-Ginglymostoma cirratum (Nurse shark) (Squalus cirratus) rag1 Polyclonal antibody
Uniprot No.
Target Names
rag1
Alternative Names
rag1 antibody; V(D)J recombination-activating protein 1 antibody; RAG-1) [Includes: Endonuclease RAG1 antibody; EC 3.1.-.-); E3 ubiquitin-protein ligase RAG1 antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase RAG1)] antibody; Fragment antibody
Raised in
Rabbit
Species Reactivity
Ginglymostoma cirratum (Nurse shark) (Squalus cirratus)
Immunogen
Recombinant Ginglymostoma cirratum (Nurse shark) (Squalus cirratus) rag1 protein
Immunogen Species
Ginglymostoma cirratum (Nurse shark) (Squalus cirratus)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
Subcellular Location
Nucleus.
Protein Families
RAG1 family
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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