Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) rag1 Polyclonal antibody

Uniprot No.

O13033

Target Names

rag1

Alternative Names

rag1 antibody; V(D)J recombination-activating protein 1 antibody; RAG-1) [Includes: Endonuclease RAG1 antibody; EC 3.1.-.-); E3 ubiquitin-protein ligase RAG1 antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase RAG1)] antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) rag1 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

Gene References into Functions

Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations PMID: 28910320
first report of any rag1(-/-) mutant vertebrate mounting a protective secondary immune response to a bacterial pathogen PMID: 22970222
target-selected inactivation of the rag1 gene PMID: 12098699
Rag1 has a role in developing zebrafish cortex-medullary regionalization PMID: 12203724
by embryonic day 4, rag1 transcripts are seen in the pancreas PMID: 12370418
Recombination signal sequences are recognized by rag1 protein. PMID: 15829313
Rag1 expression is restricted to a subpopulation of zebrafish olfactory neurons projecting to the lateral olfactory bulb; Rag11 catalytic activity is not essential for axon targeting PMID: 16018818
This report describes the leukocyte populations in a unique model, recombination activation gene 1-/- mutant zebrafish (rag1 mutants). PMID: 19192305

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Subcellular Location

Nucleus.

Protein Families

RAG1 family

Tissue Specificity

In the adult, predominantly expressed in the thymus with lower levels in the pronephros, mesonephros and ovary.

Database Links

KEGG: dre:30663

STRING: 7955.ENSDARP00000120100

UniGene: Dr.133607

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rag1 Antibody

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