RAP2-2 Antibody

Code CSB-PA885447XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAP2-2 Polyclonal antibody
Uniprot No.
Target Names
RAP2-2
Alternative Names
RAP2-2 antibody; ERF075 antibody; At3g14230 antibody; MLN21.1 antibody; Ethylene-responsive transcription factor RAP2-2 antibody; AtRAP2.2 antibody; Protein RELATED TO APETALA2 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAP2-2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcription factor involved in carotenoid biosynthesis regulation. Binds to the 5'-ATCTA-3' element present in the promoter of phytoene synthase (PSY) and phytoene desaturase (PDS). Involved in ethylene response and resistance to necrotrophic pathogens. Acts as a downstream regulator in the ethylene signaling pathway. Partially redundant with RAP2-12.
Gene References into Functions
  1. ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF75] PMID: 28164334
  2. An evolutionarily conserved 12-bp cis-regulatory motif that binds to and is sufficient for activation by RAP2.2 and RAP2.12 is identified. PMID: 26668304
  3. RAP2.2, RAP2.3 and RAP2.12 play a role in modulating oxidative and osmotic stress tolerance during submergence acclimation. PMID: 25847219
  4. RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses. PMID: 22530619
  5. Ethylene and RAP2.2, together with oxygen-dependent signal transduction, play an important role in the response to hypoxia. PMID: 20357136
  6. The action of SINAT2 may explain the recalcitrance of AtRAP2.2 protein levels to change by altering AtRAP2.2 transcription. PMID: 17873090

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Constitutive in flowers, leaves, stems, and roots.
Database Links

KEGG: ath:AT3G14230

STRING: 3702.AT3G14230.1

UniGene: At.25146

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