RAV1 Antibody

Code CSB-PA693102XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAV1 Polyclonal antibody
Uniprot No.
Target Names
RAV1
Alternative Names
RAV1 antibody; At1g13260 antibody; T6J4.2AP2/ERF and B3 domain-containing transcription factor RAV1 antibody; Ethylene-responsive transcription factor RAV1 antibody; Protein RELATED TO ABI3/VP1 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAV1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Binds specifically to bipartite recognition sequences composed of two unrelated motifs, 5'-CAACA-3' and 5'-CACCTG-3'. May function as negative regulator of plant growth and development.
Gene References into Functions
  1. AtRAV1 and AtRAV2-overexpressing cotton had approximately 5% significantly longer fibers with only marginal decreases in yields under well-watered or drought stress conditions that resulted in 40-60% yield penalties and 3-7% fiber length penalties in control plants. PMID: 26706061
  2. RAV1 transcription factor plays an important role in abscisic acid signaling by modulating the expression of ABI3, ABI4, and ABI5, and that its activity is negatively affected by SnRK2s.Rav1 is phosphorylated by SnRK2 kinases. PMID: 25231920
  3. Arabidopsis RAV1, RAV2 and Rav1L transcription factors modulates drought and salt stress responses independent of abscisic acid. PMID: 25189341
  4. Results strongly suggest that RAV1 is sufficient to cause leaf senescence and it functions as a positive regulator in this process. PMID: 20826506
  5. The RAV1 B3 binding domain consists of a seven-stranded open beta-barrel and two alpha-helices located at the ends of the barrel and is similar to the structure of the noncatalytic DNA binding domain of the restriction enzyme EcoRII. PMID: 15548737
  6. Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner. PMID: 19420805

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, RAV subfamily
Tissue Specificity
Expressed in all tissues examined: Roots, rosette leaves, cauline leaves, inflorescence stems, flowers and siliques. Highest expression in roots and rosette leaves. Very low expression in flowers.
Database Links

KEGG: ath:AT1G13260

STRING: 3702.AT1G13260.1

UniGene: At.20558

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