Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans rab-11.1 Polyclonal antibody

Uniprot No.

O01803

Target Names

rab-11.1

Alternative Names

rab-11.1 antibody; F53G12.1 antibody; Ras-related protein rab-11.1 antibody; Rab GTPase rab-11.1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans rab-11.1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Involved in regulating the meiotic maturation of oocytes. Plays a role in egg shell formation, regulating exocytosis of chondroitin proteoglycans following fertilization. Controls cortical granule localization and targets them to the plasma membrane for exocytosis. Acts as a major regulator of membrane delivery during cytokinesis. Regulates the cytoskeleton by facilitating astral microtubule elongation and organization during metaphase to ensure proper spindle alignment and polarity in the first embryonic cell division. Maintains normal endoplasmic reticulum morphology during metaphase. Involved in vesicle formation and plasma membrane repair following exposure to pore forming toxins. Regulates endocytic recycling. May play a role in yolk receptor endocytosis in growing oocytes. Plays a role in the shedding of pathogen spores from intestinal cells via its involvement in spore fusion and endocytic trafficking.

Gene References into Functions

These results suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos. PMID: 26506309
We show that the small GTPase RAB-11 localizes to apical microsporidia spores, is required for spore-containing compartments to fuse with the apical plasma membrane, and is required for spore exit. PMID: 24843160
Data suggest that PAR-5 acts as a regulatory hub for a polarity-maintaining network required for apicobasal asymmetry of F-actin and proper Rab11-RE positioning. PMID: 22634595
RAB-5 and RAB-11 are required for plasma membrane repair after attack by bacterial pore-forming toxin. PMID: 21320697
RAB-11, in conjunction with the endoplasmic reticulum, acts to regulate cell cycle-specific changes in astral microtubule length to ensure proper spindle alignment in Caenorhabditis elegans early embryos. PMID: 18385514
These results indicate that at least part of the essential role for RAB-11 and SYN-4 in early embryogenesis is in the targeting of cortical granules to the plasma membrane during the precisely regulated secretion of extracellular matrix components. PMID: 18765566

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle. Endosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Apical cell membrane. Cytoplasm, cytosol. Recycling endosome membrane; Lipid-anchor. Golgi apparatus membrane. Cytoplasmic granule.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Expressed weakly in sperm, but more predominantly in oocytes. Expressed in the intestine.

Database Links

KEGG: cel:CELE_F53G12.1

STRING: 6239.F53G12.1.2

UniGene: Cel.17482

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rab-11.1 Antibody

Product Details

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Target Background