rbfA Antibody

Datasheet

Code	CSB-PA358969XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rbfA Polyclonal antibody

Uniprot No.

P0A7G2

Target Names

rbfA

Alternative Names

rbfA antibody; P15B antibody; yhbB antibody; b3167 antibody; JW3136 antibody; 30S ribosome-binding factor antibody; Protein P15B antibody; Ribosome-binding factor A antibody; RbfA antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rbfA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S subunit. Essential for efficient processing of pre-16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Probably interacts with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses a cold-sensitive C23U 16S rRNA mutation. Overexpression decreases the lag time following cold-shock by about half, leading to faster adaptation and increased protein synthesis. Overexpression also partially suppresses a rimM deletion mutant and partially rescues its 16S rRNA processing deficiency. Its function overlaps that of Era in ribosome biogenesis. A number of RbfA mutants suppress RsgA/YjeQ deletions, in all cases less RbfA is bound to the 30S ribosome. Released from 30S ribosomes by RsgA; stimulates the ribosome-associated GTPase activity of RsgA.

Gene References into Functions

Results shows that YjeQ promotes the release of RbfA through the C-terminal helix in its zinc-finger domain. PMID: 25904134

Subcellular Location

Cytoplasm.

Protein Families

RbfA family

Database Links

KEGG: ecj:JW3136

STRING: 316385.ECDH10B_3341

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rbfA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background