RBG7 Antibody

Code CSB-PA590368XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RBG7 Polyclonal antibody
Uniprot No.
Target Names
RBG7
Alternative Names
RBG7 antibody; CCR2 antibody; GR-RBP7 antibody; GRP7 antibody; At2g21660 antibody; F2G1.7 antibody; Glycine-rich RNA-binding protein 7 antibody; AtGR-RBP7 antibody; AtRBG7 antibody; Glycine-rich protein 7 antibody; AtGRP7 antibody; Protein COLD antibody; CIRCADIAN RHYTHM antibody; AND RNA BINDING 2 antibody; Protein CCR2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RBG7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role in RNA transcription or processing during stress. Binds RNAs and DNAs sequence with a preference to single-stranded nucleic acids. Displays strong affinity to poly(U) and poly(G) sequence. Involved in mRNA alternative splicing of numerous targets by modulating splice site selection. Negatively regulates the circadian oscillations of its own transcript as well as RBG8 transcript. Forms an interlocked post-transcriptional negative feedback loop with the RBG8 autoregulatory circuit. Both proteins negatively autoregulate and reciprocally crossregulate by binding to their pre-mRNAs and promoting unproductive splicing coupled to degradation via the NMD pathway. Involved in the regulation of abscisic acid and stress responses. Affects the growth and stress tolerance under high salt and dehydration stress conditions, and also confers freezing tolerance, particularly via the regulation of stomatal opening and closing in the guard cells. Exhibits RNA chaperone activity during the cold adaptation process. Involved in the export of mRNAs from the nucleus to the cytoplasm under cold stress conditions. Target of the Pseudomonas syringae type III effector HopU1, which could probably be involved in plant innate immunity. Component of the flowering autonomous pathway which promotes floral transition, at least partly by down-regulating FLC.
Gene References into Functions
  1. Identification of a set of high-confidence targets of AtGRP7, some of which are regulated by AtGRP7 at the level of steady-state abundance or splicing. PMID: 29084609
  2. AtJAC1 coordinates with GRP7 in shaping plant development through the regulation of RNA processing in Arabidopsis. PMID: 26392261
  3. Truncation of the glycine-rich domain reduces the binding affinity of AtGRP7, showing for the first time that the glycine-rich stretch of a plant hnRNP-like protein contributes to binding. PMID: 25251471
  4. GRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs. PMID: 25104024
  5. AtGRP7 has both salicylic acid (SA)-dependent and SA-independent effects on plant immunity. PMID: 23961939
  6. AtGRP7-overexpressing plants flower with fewer leaves than wild-type plants, suggesting that the floral promotive effect of AtGRP7 bypasses the effect of a reduced gibberellin level. PMID: 24281950
  7. Data indicate that AtGRP2 and AtGRP7 affected the expression of drought stress-responsive genes in transgenic rice. PMID: 24268168
  8. AtGRP7 is a potent regulator in plant defense response to diverse pathogens, and suggest that the regulation of RNA metabolism by RNA-binding proteins is important for plant innate immunity. PMID: 22902796
  9. Proposes the first circadian slave oscillator model by studying AtGRP7 and AtGRP8 oscillations in Arabidopsis thaliana. PMID: 23555221
  10. The results show that the glycine-rich RNA-binding protein AtGRP7 influences alternative splicing in Arabidopsis thaliana. PMID: 23042250
  11. In vivo, expression of GRP7 with Arg-49 replaced with lysine does not complement the reduced immune responses of the Arabidopsis thaliana grp7-1 mutant demonstrating the importance of this residue for GRP7 function. PMID: 22013065
  12. Domain sequences encompassing the RNA recognition motif and the overall structure of GRPs (AtGRP4 harboring no RNA chaperone activity and AtGRP7 harboring such activity) are critical to chaperone activity during the cold adaptation process in cells. PMID: 21511907
  13. Involved in the regulation of abscisic acid and stress responses. PMID: 17001447
  14. A. thaliana knockout lines defective in the glycine-rich RNA-binding protein GRP7 (also known as AtGRP7), a HopU1 substrate, were more susceptible than wild-type plants to P. syringae PMID: 17450127
  15. Data show that site-specific mutation of one conserved arginine to glutamine within the RNA recognition motif impairs binding of recombinant AtGRP7 to its pre-mRNA in vitro. PMID: 17924945
  16. GRP7 affects the growth and stress tolerance of Arabidopsis plants under high salt and dehydration stress conditions, and also confers freezing tolerance, particularly via the regulation of stomatal opening and closing in the guard cells. PMID: 18410480
  17. The RNA-binding protein AtGRP7 promotes flowering, partly by downregulating FLC. Insertion mutants are late-flowering but vernalization overrides the late-flowering phenotype. PMID: 18573194
  18. AtGRP7 itself is part of a negative autoregulatory circuit that influences circadian oscillations of its own and the AtGRP8 transcript through alternative splicing linked to nonsense-mediated decay. PMID: 18987006

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Subcellular Location
Cytoplasm. Nucleus. Note=Shuttling between nucleus and cytoplasm. Relocalization from the cytoplasm into the nucleus is mediated by TRN1.
Protein Families
GR-RBP family
Tissue Specificity
Ubiquitous with strong expression in guard cell.
Database Links

KEGG: ath:AT2G21660

STRING: 3702.AT2G21660.1

UniGene: At.22672

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