RBOHD Antibody

Code CSB-PA887766XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RBOHD Polyclonal antibody
Uniprot No.
Target Names
RBOHD
Alternative Names
RBOHD antibody; At5g47910 antibody; MCA23.25 antibody; Respiratory burst oxidase homolog protein D antibody; EC 1.11.1.- antibody; EC 1.6.3.- antibody; NADPH oxidase RBOHD antibody; AtRBOHD antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RBOHD protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Calcium-dependent NADPH oxidase that generates superoxide. Involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling. Might be required for ROS signal amplification during light stress.
Gene References into Functions
  1. Differential recovery between the accessions was related to the activity of three genes: RESPIRATORY BURST OXIDASE HOMOLOG D, SENESCENCE-ASSOCIATED GENE113, and ORESARA1, which function in a regulatory network involving a reactive oxygen species (ROS) burst upon desubmergence and the hormones abscisic acid and ethylene. PMID: 29891679
  2. induction of stress marker genes and reactive oxygen species (ROS)-scavenging enzyme genes under various stress conditions is dependent on the ERF74-RbohD-ROS signal pathway. PMID: 28164334
  3. The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohF. PMID: 26765289
  4. RBOHD role in the systemic response to salt stress PMID: 27261066
  5. differential spatio-temporal expression of the Rboh genes contributes to fine-tune RBOH/NADPH oxidase-dependent reactive oxygen species production and signaling in Arabidopsis immunity. PMID: 26798024
  6. The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. PMID: 25462961
  7. Systemic acquired acclimation in plants is stress specific and that the reactive oxygen species wave is associated with the production of systemic variation potentials, specifically linking the electric signals with RBOHD function in A. thaliana. PMID: 24038652
  8. infection of Arabidopsis thaliana by the parasitic nematode Heterodera schachtii activated the NADPH oxidases RbohD and RbohF to produce ROS, which was necessary to restrict infected plant cell death and promote nurse cell formation. PMID: 24714570
  9. These findings suggest that both AtrbohD and AtrbohF are essential for abscissic acid-promoted reactive oxygen species production in roots. PMID: 23963673
  10. Mutation of Cys 890 compromised S-nitrosothiol-mediated control of AtRBOHD activity, perturbing the magnitude of cell death development. This cysteine is evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, suggesting that this mechanism may govern immune responses in both plants and animals. [RBOHD] PMID: 21964330
  11. Rhobd plays a role in the process of stomatal closure in response to salicylic acid. PMID: 23500715
  12. There is no additive effect of disease symptoms or bacterial growth in the AtrbohD x Atgox1 and AtrbohD x Atgox3 double mutants when compared with individual mutants. PMID: 22751316
  13. Data suggest that specific sphingobases can induce cell death and require respiratory burst oxidase-D (RBOHD) for early reactive oxygen species induction in plants. PMID: 21856300
  14. Mild salt stress triggers a rapid increase in RbohD expression in Arabidopsis. PMID: 21205037
  15. Reactive oxygen species generated by Atrbohs are closely related to the jasmonic acid signaling pathway. PMID: 21421415
  16. RBOHD triggers death in cells that are damaged by fungal infection but simultaneously inhibits death in neighboring cells through the suppression of free salicylic acid and ethylene levels. PMID: 19726575
  17. Ca(2+) binding and phosphorylation synergistically activate the ROS-producing enzyme activity of AtrbohD. PMID: 18218618
  18. The rbohD mutant expressing non-PA-binding RbohD was compromised in ABA-mediated ROS production and stomatal closure. PMID: 19690149
  19. Data show that a rapid systemic signal that traveled at a rate of 8.4 centimeters per minute and was dependent on the respiratory burst oxidase homolog D (RbohD) gene. PMID: 19690331

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
RBOH (TC 5.B.1.3) family
Tissue Specificity
More abundant in roots than in leaves, stems or inflorescences. Expressed in mesophyll and guard cells.
Database Links

KEGG: ath:AT5G47910

STRING: 3702.AT5G47910.1

UniGene: At.23270

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