RBOHJ Antibody

Code CSB-PA864905XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RBOHJ Polyclonal antibody
Uniprot No.
Target Names
RBOHJ
Alternative Names
RBOHJ antibody; At3g45810 antibody; F16L2.20 antibody; Putative respiratory burst oxidase homolog protein J antibody; EC 1.11.1.- antibody; EC 1.6.3.- antibody; NADPH oxidase RBOHJ antibody; AtRBOHJ antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RBOHJ protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Calcium-dependent NADPH oxidase that generates superoxide.
Gene References into Functions
  1. apoplastic reactive oxygen species derived from RbohH and RbohJ are involved in pollen tube elongation into the stigmatic papillae by affecting the cell wall metabolism.[RbohJ] PMID: 25751652
  2. RBOHH and RBOHJ were specifically expressed in pollen and dynamically targeted to distinct and overlapping plasma membrane domains at the pollen tube tip. Functional loss of RBOHH and RBOHJ in homozygous double mutants resulted in reduced fertility. PMID: 24506280
  3. Disruption of the pollen expressed NADPH oxidases RbohH and RbohJ triggers altered reproductive phenotypes. PMID: 24302886
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
RBOH (TC 5.B.1.3) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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