HRT1 Antibody

Code CSB-PA600475XA01SVG
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Product Details

Full Product Name Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HRT1 Polyclonal antibody
Uniprot No. Q08273
Target Names HRT1
Alternative Names HRT1 antibody; RBX1 antibody; ROC1 antibody; YOL133W antibody; RING-box protein HRT1 antibody; RING-box protein 1 antibody; E3 ubiquitin-protein ligase complex SCF subunit HRT1 antibody; High level expression reduces Ty3 transposition protein 1 antibody; Regulator of cullins protein 1 antibody
Raised in Rabbit
Species Reactivity Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) HRT1 protein
Immunogen Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

(From Uniprot)
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs), which mediate the ubiquitination of target proteins. Recruits the E2 ubiquitin-conjugating enzyme CDC34/UBC3 to the complex and brings it into close proximity to the substrate. Also stimulates CDC34/UBC3 autoubiquitination and promotes the neddylation of CDC53 and RTT101. Component of the SCF(CDC4) ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor SIC1 and for the G1-to-S phase transition. Component of the RTT101(MMS1-MMS22) ubiquitin ligase that promotes fork progression through damaged DNA or natural pause sites. Component of the CRL3(ELA1) ubiquitin ligase required for ubiquitination of RPB1, the largest subunit of RNA polymerase II (Pol II), which targets Pol II for proteasomal degradation in DNA-damaged cells.
Subcellular Location Cytoplasm. Nucleus.
Protein Families RING-box family
Database Links

KEGG: sce:YOL133W

STRING: 4932.YOL133W

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